11_ICP_methods
URL: https://data.rothamsted.ac.uk/dataset/4c6463e3-7500-4a65-9292-9843e4f1a764/resource/4de9e880-98a3-4375-bdca-b0a3f53d55ff/download/11_icp_methods.csv
Soil, herbage and manure samples were air-dried and finely ground prior to analysis. Total I was analysed using a 25% tetramethylammonium hydroxide (TMAH) extraction for 4 hours with analysis by ICP-MS by NUVetNA (University of Nottingham, UK). For the analysis of the other elements, soil and manure samples were prepared using an aqua regia digest (McGrath and Cunliffe 1985), and herbage samples by microwave digestion. Analysis was by either ICP-MS (NexION 300X Inductively Coupled Plasma – Mass Spectrometer, Perkin Elmer) or ICP-OES (Optima 7300 DV Inductively Coupled Plasma – Optical Emission Spectrometer, Perkin Elmer), depending on element concentrations and this table contains information on which was used for each soil type.\nUrine total I was analysed using the same method as for the other sample types, but a creatinine adjustment level was also calculated. Creatinine is a waste product of protein metabolism and is excreted in urine at a constant rate relative to muscle mass. Making the assumption that the cattle in this study have similar muscle masses, urine creatinine concentration can therefore be used as a correction against element concentrations in urine varying with hydration levels. To calculate an element relative to creatinine, multiply the creatinine adjustment value by the element concentration. For the analysis of other elements, urine samples were prepared by either aqua regia or microwave digestion and analysed by either ICP-OES or ICP-MS, as shown in this table
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